restriction enzyme sacii (New England Biolabs)
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restriction enzyme sacii
Restriction Enzyme Sacii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 862 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sac+ii+restriction+enzymes/Sac+II/10__1016_slash_j__scr__2026__103973-43-27-30
Average 96 stars, based on 862 article reviews
Restriction Enzyme Sacii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 862 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sac+ii+restriction+enzymes/Sac+II/10__1016_slash_j__scr__2026__103973-43-27-30
Average 96 stars, based on 862 article reviews
restriction enzyme sacii - by Bioz Stars,
2026-10
96/100 stars
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Construct:Article Title: Spray-induced gene silencing (SIGS) as a tool for the management of Pine Pitch Canker forest disease Article Snippet: .. For dsRNA vectors assembly, constructs sequences were synthesized in Integrated DNA Technologies (IDT, Coralville, IA, USA) and cloned into the T777T plasmid (Addgene plasmid # 113082; http://n2t.net/addgene:113082 ; RRID:Addgene_113082) which contains two IPTG-inducible T7 promoter (5’-TAATACGACTCACTATAG-3’) in opposite directions that synthesize complementary RNA strands to form dsRNAs ( ).Plasmid and inserts were digested with Kpn I and BglII or Article Title: A piRNA-lncRNA regulatory network initiates responder and trailer piRNA formation during mosquito embryonic development Article Snippet: .. To generate reporter constructs, a pMT-GL3 vector, encoding firefly luciferase (GL3) under control of a copper sulphate (CuSO 4 )-inducible metallothionein promotor (pMT) , was digested with Pme I and Article Title: Intra- and extra-cellular environments contribute to the fate of HIV-1 infection Article Snippet: Sequences required for Gibson Assembly were added to the fragments during amplification and the three DNA fragments were cloned into the pNL4-3.HSA.R-E-plasmid, which was digested with Not I and Xho I restriction enzymes (NEB), using Gibson Assembly (NEbuilder; NEB). .. All subsequent HI.fate vectors containing different internal promoters were constructed by digesting HI.fate.E with BsiW I and Article Title: Intra- and extra-cellular environments contribute to the fate of HIV-1 infection Article Snippet: R − E − plasmid, which was digested with Not I and Xho I restriction enzymes (NEB), using Gibson Assembly (NEbuilder; NEB). .. All subsequent HI.fate vectors containing different internal promoters were constructed by digesting HI.fate.E with BsiW I and Synthesized:Article Title: Spray-induced gene silencing (SIGS) as a tool for the management of Pine Pitch Canker forest disease Article Snippet: .. For dsRNA vectors assembly, constructs sequences were synthesized in Integrated DNA Technologies (IDT, Coralville, IA, USA) and cloned into the T777T plasmid (Addgene plasmid # 113082; http://n2t.net/addgene:113082 ; RRID:Addgene_113082) which contains two IPTG-inducible T7 promoter (5’-TAATACGACTCACTATAG-3’) in opposite directions that synthesize complementary RNA strands to form dsRNAs ( ).Plasmid and inserts were digested with Kpn I and BglII or Article Title: Intra- and extra-cellular environments contribute to the fate of HIV-1 infection Article Snippet: Sequences required for Gibson Assembly were added to the fragments during amplification and the three DNA fragments were cloned into the pNL4-3.HSA.R-E-plasmid, which was digested with Not I and Xho I restriction enzymes (NEB), using Gibson Assembly (NEbuilder; NEB). .. All subsequent HI.fate vectors containing different internal promoters were constructed by digesting HI.fate.E with BsiW I and Article Title: Intra- and extra-cellular environments contribute to the fate of HIV-1 infection Article Snippet: R − E − plasmid, which was digested with Not I and Xho I restriction enzymes (NEB), using Gibson Assembly (NEbuilder; NEB). .. All subsequent HI.fate vectors containing different internal promoters were constructed by digesting HI.fate.E with BsiW I and Clone Assay:Article Title: Spray-induced gene silencing (SIGS) as a tool for the management of Pine Pitch Canker forest disease Article Snippet: .. For dsRNA vectors assembly, constructs sequences were synthesized in Integrated DNA Technologies (IDT, Coralville, IA, USA) and cloned into the T777T plasmid (Addgene plasmid # 113082; http://n2t.net/addgene:113082 ; RRID:Addgene_113082) which contains two IPTG-inducible T7 promoter (5’-TAATACGACTCACTATAG-3’) in opposite directions that synthesize complementary RNA strands to form dsRNAs ( ).Plasmid and inserts were digested with Kpn I and BglII or Plasmid Preparation:Article Title: Spray-induced gene silencing (SIGS) as a tool for the management of Pine Pitch Canker forest disease Article Snippet: .. For dsRNA vectors assembly, constructs sequences were synthesized in Integrated DNA Technologies (IDT, Coralville, IA, USA) and cloned into the T777T plasmid (Addgene plasmid # 113082; http://n2t.net/addgene:113082 ; RRID:Addgene_113082) which contains two IPTG-inducible T7 promoter (5’-TAATACGACTCACTATAG-3’) in opposite directions that synthesize complementary RNA strands to form dsRNAs ( ).Plasmid and inserts were digested with Kpn I and BglII or Article Title: Novel Mutations in the TMPRSS3 Gene May Contribute to Taiwanese Patients with Nonsyndromic Hearing Loss Article Snippet: The RT-PCR products were cloned into an pGEM-T Easy Vector according to the manufacturer’s recommendations (Promega, Madison WI). .. The three subunits were then subcloned into the pTLN vector using Hind III and Article Title: Intra- and extra-cellular environments contribute to the fate of HIV-1 infection Article Snippet: Sequences required for Gibson Assembly were added to the fragments during amplification and the three DNA fragments were cloned into the pNL4-3.HSA.R-E-plasmid, which was digested with Not I and Xho I restriction enzymes (NEB), using Gibson Assembly (NEbuilder; NEB). .. All subsequent HI.fate vectors containing different internal promoters were constructed by digesting HI.fate.E with BsiW I and Article Title: Fluconazole resistance and CDR1 expression in Candida albicans mediated by the hyperactive Tac1-5 transcriptional activator requires Tlo proteins Article Snippet: .. The plasmid was linearized for transformation purposes using Kpn I and Article Title: Intra- and extra-cellular environments contribute to the fate of HIV-1 infection Article Snippet: R − E − plasmid, which was digested with Not I and Xho I restriction enzymes (NEB), using Gibson Assembly (NEbuilder; NEB). .. All subsequent HI.fate vectors containing different internal promoters were constructed by digesting HI.fate.E with BsiW I and Luciferase:Article Title: A piRNA-lncRNA regulatory network initiates responder and trailer piRNA formation during mosquito embryonic development Article Snippet: .. To generate reporter constructs, a pMT-GL3 vector, encoding firefly luciferase (GL3) under control of a copper sulphate (CuSO 4 )-inducible metallothionein promotor (pMT) , was digested with Pme I and Control:Article Title: A piRNA-lncRNA regulatory network initiates responder and trailer piRNA formation during mosquito embryonic development Article Snippet: .. To generate reporter constructs, a pMT-GL3 vector, encoding firefly luciferase (GL3) under control of a copper sulphate (CuSO 4 )-inducible metallothionein promotor (pMT) , was digested with Pme I and Cloning:Article Title: Intra- and extra-cellular environments contribute to the fate of HIV-1 infection Article Snippet: Sequences required for Gibson Assembly were added to the fragments during amplification and the three DNA fragments were cloned into the pNL4-3.HSA.R-E-plasmid, which was digested with Not I and Xho I restriction enzymes (NEB), using Gibson Assembly (NEbuilder; NEB). .. All subsequent HI.fate vectors containing different internal promoters were constructed by digesting HI.fate.E with BsiW I and Article Title: Intra- and extra-cellular environments contribute to the fate of HIV-1 infection Article Snippet: R − E − plasmid, which was digested with Not I and Xho I restriction enzymes (NEB), using Gibson Assembly (NEbuilder; NEB). .. All subsequent HI.fate vectors containing different internal promoters were constructed by digesting HI.fate.E with BsiW I and Blocking Assay:Article Title: Intra- and extra-cellular environments contribute to the fate of HIV-1 infection Article Snippet: Sequences required for Gibson Assembly were added to the fragments during amplification and the three DNA fragments were cloned into the pNL4-3.HSA.R-E-plasmid, which was digested with Not I and Xho I restriction enzymes (NEB), using Gibson Assembly (NEbuilder; NEB). .. All subsequent HI.fate vectors containing different internal promoters were constructed by digesting HI.fate.E with BsiW I and Article Title: Intra- and extra-cellular environments contribute to the fate of HIV-1 infection Article Snippet: R − E − plasmid, which was digested with Not I and Xho I restriction enzymes (NEB), using Gibson Assembly (NEbuilder; NEB). .. All subsequent HI.fate vectors containing different internal promoters were constructed by digesting HI.fate.E with BsiW I and Transformation Assay:Article Title: Fluconazole resistance and CDR1 expression in Candida albicans mediated by the hyperactive Tac1-5 transcriptional activator requires Tlo proteins Article Snippet: .. The plasmid was linearized for transformation purposes using Kpn I and Sequencing:Article Title: Development of a Bicistronic Vector for the Expression of a CRISPR/Cas9-mCherry System in Fish Cell Lines Article Snippet: The expression vector LentiCRISPR-Cas9-2A-mCherryU6ZF (LcU6ZF, hereafter) created for fish cell lines was based on the mammalian LentiCRISPR Puro V2 from Feng Zhang ́s lab, (addgene plasmid #52961) [ ] which was modified in two steps, as follows. .. To generate LCmCherry V2, the mCherry sequence was obtained from FU-mCherry-w (derived from FUGW) [ ] and then digested with Bsi WI and Derivative Assay:Article Title: Development of a Bicistronic Vector for the Expression of a CRISPR/Cas9-mCherry System in Fish Cell Lines Article Snippet: The expression vector LentiCRISPR-Cas9-2A-mCherryU6ZF (LcU6ZF, hereafter) created for fish cell lines was based on the mammalian LentiCRISPR Puro V2 from Feng Zhang ́s lab, (addgene plasmid #52961) [ ] which was modified in two steps, as follows. .. To generate LCmCherry V2, the mCherry sequence was obtained from FU-mCherry-w (derived from FUGW) [ ] and then digested with Bsi WI and |